Abstract
High parameter single cell analysis has driven deep understanding of immune processes. Using a next-generation single-cell “mass cytometry” platform, the speaker quantified surface and cytokine or drug responsive indices of kinase target with 45 or more parameter analysis (e.g. 45 antibodies, viability, nucleic acid content and relative cell size). Similarly, he has developed two advanced technologies that enable deep phenotyping of solid tissue in both fresh frozen and FFPE formats (50 – 100 markers). He has recently extended this parameterization to mRNA with the capability to measure down to 5 molecules per cell in combination with any other set of previously created markers.
In this lecture, he will present evidence of deep internal order in immune functionality demonstrating that differentiation and immune activities have evolved with a definable “shape”. This shape is altered during immune surveillance and “imprinted” during, and after, pathogen attack, traumatic injury, or auto-immune disease. Hierarchies of functionally defined trans-cellular modules are observed that can be used for mechanistic and clinical insights. He will focus upon pathogen attack, traumatic surgical intervention in human and auto-immune processes for the presentation.
About the speaker
Prof Garry Nolan received his BS in Biology from Cornell University in 1983 and PhD in Genetics from Stanford University in 1989. He was a postdoctoral fellow in the laboratory at Rockefeller University during 1990-1993. He then returned to Stanford as an Assistant Professor and is currently the Rachford and Carlota Harris Professor in Stanford University School of Medicine.
Prof Nolan’s research focused on signal transduction, immunology, cancer biology, auto-immunity, retroviral design, bioinformatics and genetics. His recent research interests include studying signaling alterations at the single cell level in leukemia and lymphomas, cancer stem cells, and determining which of these signaling attributes correlate with patient outcome, drug reactivity and mechanism of disease progressions.
Prof Nolan was elected the Hume Faculty Scholar from 1993 to 1998, the Trustee of the Leukemia Society of America from 1995 to 1998, the Burroughs Wellcome Fund New Investigator Awardee from 1996 to 2000 and the Leukemia and Lymphoma Society Stahlman Scholar in 2000.
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